Academic Positions

  • 2014 2009

    Head of Department

    Ahmadu Bello University, Department of Biochemistry

  • 2010 2004

    SIWES Coordinator

    Ahmadu Bello University, Zaria

  • 2014 2009

    Chairman

    Ahmadu Bello University, Departmental Committee on Student Welfare

  • 2014 2009

    Chairman

    Ahmadu Bello University, Departmental Committee on Instruments and Environment

  • Other Positions Held

Education & Training

  • Ph.D. 1989

    Ph.D. in Biochemistry

    Ahmadu Bello University, Zaria

  • M.B.A.1979

    Master of Science Biochemistry

    Ahmadu Bello University, Zaria

  • B.Sc.1975

    Bachelor of Science in Biochemistry

    Ahmadu Bello University, Zaria

Awards

  • 1998
    Evans Medical PLC
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    Development of pregelatinised maize starch as tablet excipient.
  • 1986-1989
    Commonwealth Academic Staff Award
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    Recipient of Commonwealth Academic Staff Award.
  • 1997-1979
    Scholarship Award
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    University Scholarship for MSc programme.
  • 1975
    University Scholar
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    BSc (Hons) Second Class, Upper. Best Student of the year 1975. University Scholar.
  • 1969
    Highest Scores, Kano State
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    The only Grade One in WAEC examination in Kano State.

Laboratory Personel

David A. Doe

Postdoctoral fellow

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James Doe

Postdoctoral fellow

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Nadja Sriram

Postdoctoral fellow

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Davide Doe

Research Assistant

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Pauline Doe

Summer Intern

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James Doe

Postdoctoral fellow

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Great lab Personel!

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Research Projects

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Characterization of DNA Methylase from Peas.

H.M.I. YESUFU, R.L.P. ADAMS
Conference Papers Journal of Cellular Biochemistry. Supplement 13D, p 216, UCLA Symposia on Mol. and Cell. Biology

Abstract

DNA methylase activity was detected in nuclei from pea shoots. The enzyme can only be extracted by low-salt treatment if the nuclei are pretreated with micrococcal nuclease. Only a single enzyme was detected, and it was purified to a specific activity of 1620 units/mg of protein. It has an Mr of 160,000 on gel filtration and SDS/PAGE. Pea DNA methylase methylates cytosine in all four dinucleotides, and this is interpreted to show that it acts on CNG trinucleotides. Although it shows a strong preference for hemi-methylated double-stranded DNA, it is also capable of methylation de novo. Homologous DNA is the best natural substrate. In vitro the enzyme interacts with DNA to form a salt-resistant complex with DNA that is stable for at least 4 h.

Hypovitaminosis A and Measles Infection in Pediatric Outpatients at ABU Teaching Hospital: A Tribute to Professor Clive West.

H.M. Inuwa, H.O. Akanya
Conference PapersBook of Abstracts, 24th Annual Conference of the Nigerian Society of Biochemistry and Molecular Biology, p: 28

Abstract

This work was prompted by the observation that corneal ulceration was apparently more common in young children in the guinea savannah area of northern Nigeria than in children in the tropical rainforest areas of southern Nigeria, where the intake of vitamin A, as the provitamin in red palm oil, is higher. Since corneal ulceration was usually seen in association with measles, a study was carried out to clarify the relationship between nutritional status, measles infection.

Cloning and Expression of Sialidase genes from Trypanosoma congolense (STB212) and Trypanosoma vivax (AGAI strain)

H.M. Inuwa, K.A.N. Esievo, A.J. Nok
Book of Abstracts, 25th Annual Conference of the Nigerian Society of Biochemistry and Molecular Biology, p: 22

Abstract

Trans-sialidase (TS) is a unique enzyme with glycosyltransferase and sialidase (SA) activities. Although many authors reported that TS is not found in the blood stream form (BSF) of African trypanosomes, SA activity has been observed in the sera of infected animals and the BSF parasites, contributing to initial and continuous anaemia. In this study, a polymerase chain reaction (PCR) based approach was used to detect a trans-sialidase gene from the bloodstream form of Trypanosoma evansi (Te) obtained from the blood of infected camels. Sequence analysis of the cloned TeTS gene indicated 99% identity to some African trypanosomes trans-sialidase genes. Unique amino acids motifs found to occur in all African trypanosomes TS genes were identified in the TeTS gene. Catalytic site residues common to SA and TS genes were identified on the catalytic region of the gene. These results indicate that TeTS is homologous to TS gene sequences and thus strongly suggests the occurrence of TS in the BSF of T. evansi.

Isolation and Characterisation of Mannosylated Lectin from Garlic (Allium sativum)

Mairo Inuwa, N.B. Afolabi-Balogun , A.J. Nok
Book of Abstracts, 25th Annual Conference of the Nigerian Society of Biochemistry and Molecular Biology, p: 22

Abstract

Monocot mannose-binding lectins (MMBLs) or agglutinins are an extended superfamily of structurally and evolutionarily related proteins. They play important roles in plant defenses. Here we describe the synthesis of full-length cDNA of monocot mannose-binding insecticidal agglutinin isolated from Allium sativum, a traditional herb known to be of great applications in Africa, using reverse transcription polymerase chain reaction (RT-PCR) with specific primers designed based on the insecticidal sequence (NCBI primary accession no. AY866499.1). Sequence analysis revealed a 327bp open reading frame (ORF) encoding a putative 108-aa agglutinin precursor with a C-terminal domain. Multiple alignments of BLEC1 amino acids with those of eight other MMBLs revealed three highly conserved domains among them, indicating BLEC1 belongs to a member of the MMBL superfamily. Tertiary structure analysis showed that BLEC1 had three potential equal mannose-binding sites. Phylogenetic analysis indicated that 20 MMBLs including BLEC1 belonged to an extended superfamily. Gene ontology analyses indicate one biological process with GO ID: 0006952 representing defense response, with two secondary IDs GO: 0002217 GO: 0042829. The child terms has both negative and positive regulation some of which include GO: 0002242 defense response to parasitic plant and GO: 0002213 defense response to insect. The cloning and characterization of BLEC1 will enable us to study its potential use in plant genetic engineering in the development of insect resistance plant.

Isolation and Characterisation of Mannosylated Lectin from Garlic (Allium sativum)

Hajiya Mairo Inuwa, Aimola Idowu Asegame, Nok Andrew Jonathan, Mamman Aisha
Isolation of the Beta-Globin Gene Cluster Fragment from a Nigerian SS Genotype

Abstract

Monocot mannose-binding lectins (MMBLs) or agglutinins are an extended superfamily of structurally and evolutionarily related proteins. They play important roles in plant defenses. Here we describe the synthesis of full-length cDNA of monocot mannose-binding insecticidal agglutinin isolated from Allium sativum, a traditional herb known to be of great applications in Africa, using reverse transcription polymerase chain reaction (RT-PCR) with specific primers designed based on the insecticidal sequence (NCBI primary accession no. AY866499.1). Sequence analysis revealed a 327bp open reading frame (ORF) encoding a putative 108-aa agglutinin precursor with a C-terminal domain. Multiple alignments of BLEC1 amino acids with those of eight other MMBLs revealed three highly conserved domains among them, indicating BLEC1 belongs to a member of the MMBL superfamily. Tertiary structure analysis showed that BLEC1 had three potential equal mannose-binding sites. Phylogenetic analysis indicated that 20 MMBLs including BLEC1 belonged to an extended superfamily. Gene ontology analyses indicate one biological process with GO ID: 0006952 representing defense response, with two secondary IDs GO: 0002217 GO: 0042829. The child terms has both negative and positive regulation some of which include GO: 0002242 defense response to parasitic plant and GO: 0002213 defense response to insect. The cloning and characterization of BLEC1 will enable us to study its potential use in plant genetic engineering in the development of insect resistance plant

Induction of Protective Immunity by Prime-boosting Immunization wth Cysteine protease as Antigen against Plasmodium bergei (Anka strain)

Hajiya Mairo Inuwa, Amlabu E., Nok A. J., Inuwa H.M., Umar I.A., Akin-Osanaiye B.C. Haruna E.
Book of Abstracts, 27th Annual Conference of the Nigerian Society of Biochemistry and Molecular Biology, p: 83.

Abstract

Crude preparations of malaria parasite proteins from the hydrophobic and hydrophilic phases were made by Triton X-100 temperature-induced phase separation procedures. Cysteine protease was purified from the hydrophobic phase of the parasite protein preparation. Groups of BALB/c mice were immunized intraperitoneally with the purified cysteine protease and crude preparations of the parasite proteins respectively. Priming and booster immunizations were administered on days 0, 14 and 21 prior to lethal parasite challenge on day 30. The course of infection was monitored by microscopic Giemsa-stained, thin blood smears. Protection was conferred at varying thresholds in the groups of mice immunized. This was shown by a week delay in the onset of parasitemia and red blood cell invasion by parasites in the group of mice immunized with the purified cysteine protease. Pack Cell Volume, parasite burden and the mean survival time of mice in days post-infection when compared to experimental controls indicated that protection was conferred in mice during immunization. Our data shows that the parasite enzyme cysteine protease is a potential target which can further be exploited for precise drug targeting and vaccine development against malaria.

Induction of Protective Immunity by Prime-boosting Immunization wth Cysteine protease as Antigen against Plasmodium bergei (Anka strain)

H.M. Inuwa, Nok A. J., Ado S. G., Usman I. S. and Halilu A. D.
Book of Abstracts, 27th Annual Conference of the Nigerian Society of Biochemistry and Molecular Biology, p: 83.

Abstract

Crude preparations of malaria parasite proteins from the hydrophobic and hydrophilic phases were made by Triton X-100 temperature-induced phase separation procedures. Cysteine protease was purified from the hydrophobic phase of the parasite protein preparation. Groups of BALB/c mice were immunized intraperitoneally with the purified cysteine protease and crude preparations of the parasite proteins respectively. Priming and booster immunizations were administered on days 0, 14 and 21 prior to lethal parasite challenge on day 30. The course of infection was monitored by microscopic Giemsa-stained, thin blood smears. Protection was conferred at varying thresholds in the groups of mice immunized. This was shown by a week delay in the onset of parasitemia and red blood cell invasion by parasites in the group of mice immunized with the purified cysteine protease. Pack Cell Volume, parasite burden and the mean survival time of mice in days post-infection when compared to experimental controls indicated that protection was conferred in mice during immunization. Our data shows that the parasite enzyme cysteine protease is a potential target which can further be exploited for precise drug targeting and vaccine development against malaria.

Validation of HPLC-Method for Rapid Determination of a-Tocopherol

H.M. Inuwa, I. A. Aimola, A.Magomya, Z. R. Manshak, Z. Ladan, A. S. Agbaji,
Book of Abstracts, Nigerian Society of Biochemistry and Molecular Biology, North West Zonal Conference, Zaria. pp: 17

Abstract

Abstract Available on Request.

Endosperm Proteins Profiles as Indicator of Protein Quality In Maize

H.M. Inuwa, A. D. Halilu, H. M. Inuwa, A. J. Nok, H. C. Nzelibe
Nigerian Society of Biochemistry and Molecular Biology. North West Zonal Conference. pp: 20.

Abstract

Abstract Available on Request

Comparative Analysis of Genomic DNA Isolation Procedures; Hexadecyltrimethylamonium bromide (CTAB) and Liquid Detergent (Morning Fresh) Methods From Samsorg 41 (ICSV 400)

H.M. Inuwa, Aimola, I. A. and Inuwa, L.
Book of Abstracts, IITA 1st IARSAF International Conference, Ibadan. Pp: 53.

Abstract

Abstract Available on Request

The Effect of Measles Infection and Malnutrition on plasma retinol (Vitamin A) and albumin concentration in children in Zaria.

M. Inua2
Science Association of Nigeria Conference, Port Harcourt.

Abstract

Abstract Available on Request

Nuclear DNA Methylase from Pisum sativum.

H. M. I. Yesufu
Biochemical Society Conference, Sheffield.

Abstract

DNA methylase activity was detected in nuclei from pea shoots. The enzyme can only be extracted by low-salt treatment if the nuclei are pretreated with micrococcal nuclease. Only a single enzyme was detected, and it was purified to a specific activity of 1620 units/mg of protein. It has an Mr of 160,000 on gel filtration and SDS/PAGE. Pea DNA methylase methylates cytosine in all four dinucleotides, and this is interpreted to show that it acts on CNG trinucleotides. Although it shows a strong preference for hemi-methylated double-stranded DNA, it is also capable of methylation de novo. Homologous DNA is the best natural substrate. In vitro the enzyme interacts with DNA to form a salt-resistant complex with DNA that is stable for at least 4 h.

Characterization of DNA Methylase from Peas.

H. M. I. Yesufu
UCLA Symposia on Molecular and Cellular Biology, Frisco, Colorado.

Abstract

Sourcing of Pharmaceutical Raw Materials

H.M. Inuwa
Journal of National Institute for Pharmaceutical Research and Development (NIPRD). 1994: 5–6

Abstract

Fractionation of Genomic DNA from Gossypium hirsutum L. (Multiadversity Resistant & Samcot 10’) Using EcoRI and HindIII

H.M. Inuwa S. A. Alabi, J. N. Nok, E. O. Balogun, Chiezey, O. M
19th Annual Conference of Biotechnology Society of Nigeria, Jos.

Abstract

Isolation of Megabase Genomic DNA and Restriction Fragment of the Beta-Globin Gene Cluster from Three human Genotype

Hajia Mairo Inuwa
27th Annual Conference of the Nigerian Society of Biochemistry and Molecular Biology, FUTO, Owerri.

Abstract

Genotyping of Drought Tolerant Maize Germplasm of NARS (IAR)

H. M. Inuwa
Planning Meeting of Drought Tolerant Maize for Africa (DTMA) Project. IITA, Ibadan.

Abstract

Post Measles Corneal Ulceration in Children in Northern Nigeria,The role of vitamin A, Malnutrition and Measles

M. Inua, M. B Duggan, C. E. West, H.C. Whittle, O. I. Kogbe, J. H. Sandford-Smith, J. Glover
Journal Paper Annals of Tropical Paediatrics, 3: 181 – 191.

Abstract

Acute corneal ulceration in malnourished children is the commonest cause of childhood blindness in Northern Nigeria and usually develops after measles. Other severe diseases in malnourished children rarely precipitate corneal ulceration. A survey in a school for blind children showed that 69% of the children were blind from corneal disease, and a survey of children with corneal scars showed that at least 42% were caused by ulceration after measles. The clinical appearance of the active ulcers was very varied. The serum retinol-binding protein and prealbumin levels in children with corneal ulcers following measles were below normal, but a group of malnourished children without eye complaints following measles were found to have even lower levels. Thus a specific deficiency of vitamin A does not appear to be the primary cause of these ulcers, though it may be a contributory one. A specific measles keratitis and secondary herpes simplex infectious may be local factors contributing to this ulceration, and there is nearly always a background of protein calorie malnutrition. Racial factors may also be of some significance.

Eukaryotic Dna Methylases and Their Use for in Vitro Methylation

H.M.I Yesufu, R.L.P. Adams, M. Bryans, A. Rinaldi, A. Smart,
Journal Paper Philos. Trans. R Soc Lond. B Biol. Sci. 326: 189-198.

Abstract

DNA methylases from mouse and pea have been purified and characterized. Both are high molecular mass enzymes that show greater activity with hemimethylated than unmethylated substrate DNA. Both methylate cytosines in CpG preferentially, but not exclusively and show similar kinetics of methylation, which makes it difficult to saturate all possible sites on the DNA, but procedures are described that circumvent this problem.

DNA Methylase from Pisum Sativum.

H.M.I Yesufu<, R.L.P. Adams, M. Bryans, A. Rinaldi, A. Smart,
Journal Paper Biochemistry Journal. 273: 469-475.

Abstract

Semen Sialic acid Surge and Modulation of α-L-Fucosidase Activity: Possible link to loss in Reproductive Capacity during Trypanosomiasis.

H. Mairo INUWA, O. Oluyinka Okubanjo, A. Ajanusi; Ogwu David; V. Sekoni, Andrew J. Nok
Journal Paper Cell Biochemistry and Function. 24: 1-8.

Abstract

The profiles of semen sialic acid and the enzyme α-L-fucosiadase were studied in rams undergoing chronic infection by Trypanosoma congolense. Our data showed a significant surge in the level of sialic acid with parasitaemia. The pattern followed a polynomial function we had reported for erythrocyte sialic acid in mice undergoing acute infection by T. congolense.1 The activity of the enzyme α-fucosidase decreased progressively with approximately 60% decrease at the end of the 14 weeks of infection. Representative semen samples from the control and infected rams were subjected to kinetic characterization. While the uninfected semen sample showed two active pH peaks at 4.5–5.5 and at 6.8–7.2, respectively, there was an apparent shift to only a single pH optimum at 4.5–5.5 for the pathological semen. The fucosidases from both sources were optimally active at 35°C albeit with contrasting activation energies (Ea) with values 20.58 and 35 kJ/mol for the control and infected semen, respectively. Kinetic studies using methylumbelliferyl-β-fucoside (4MU-Fuc) as substrate gave KM and Vmax values of 3.25 µM and 14.6 µmol. min−1 mg−1, respectively for the control semen. The values for the infected semen were 18.25 µM and 10.5 µmol. min−1 mg−1, respectively. The significance of these results is discussed as they relate to loss in reproductive capacity in trypanosomoses.

Characterization of a stable Isoenzyme of Malate Dehydrogenase (MDHI) from blood Stream Trypanosoma.

H. Mairo INUWA, A. U. Wurochekke, A. J. Nok, C. E. Gimba
Journal Paper International Journal of Physical Sciences 3 (9): 224-230

Abstract

Effectiveness of Genomic DNA Extraction Protocol from Three Species of African Mistletoe (Lorantheceae).

H. Mairo INUWA, V. O. AINA, S. IBRAHIM, D. A. AMEH, A. J. NOK, I. AIMOLA, P. A. WUYEP
Journal Paper Journal of Pharmaceutical and Allied Sciences. 6 (3): 676-681.

Abstract

Molecular Studies of Three Species of African Mistletoe for Fragment Responsible for Antidiabetic Activity

H. Mairo INUWA, V. O. AINA, S. IBRAHIM
Journal Paper Journal of Pharmaceutical and Allied Sciences. 6 (2): 751-754.

Abstract

Phytochemical Screening and Antibacterial Activity of Tapinanthus dodoneifolius Extracts.

H. Mairo Inuwa, V. O. Aina, S. Ibrahim
Journal Paper Journal of Pharmaceutical and Allied Sciences. 7 (3): 994 -997

Abstract

Phytochemical Screening and Antibacterial Activity of Tapinanthus dodoneifolius Extracts.

H. M. Inuwa, D. A. Ameh, S. Ibrahim
Journal Paper Journal of Pharmaceutical and Allied Sciences. 7 (3): 994 -997

Abstract

Antidiabetic activity of Dried Leaves of Aginanthus brunneus on Alloxan-Induced Diabetic Rats.

H. M. Inuwa, D. A. Ameh, S. Ibrahim
Journal Paper Continental Journal of Biomedical Sciences. 4: 37 - 42.

Abstract

Antidiabetic activity of Dried Leaves of Aginanthus brunneus on Alloxan-Induced Diabetic Rats.

H. M. Inuwa, V. O. Aina, S. Ibrahim
Journal Paper Continental Journal of Biomedical Sciences. 4: 37 - 42.

Abstract

Focus on Sickle Cell Disease Research.

H. M. Inuwa, A. I. Mamman , A. J. Nok , E. Amlabu , U. S. Ndidi , T. K. Bello, I. A. Bello , B. Afolabi-Balogun, F. Balogun
Journal Paper International Journal of Biological Science. 2(2): 16-22.

Abstract

Rapid Targeting and Isolation of the β-like globin gene cluster fragment from AA, AS and SS genotypes using BamHI restriction enzyme.

H. M. Inuwa, Idowu Asegame Aimola, Andrew Jonathan Nok, I. Aisha Mamman
Journal Paper International Journal of Genetics and Molecular Biology. 2 (10): 202-206.

Abstract

Terminalia catappa Extract Enhances Erythropoiesis in Adult Balb C Mice.

H. M. Inuwa, A. I. Mamman, N. Habila, A. S. Agbaji, D. Omoniwa, I. A. Aimola
Journal Paper Journal of Molecular Biology Research 1(1): 40- 46.

Abstract

Comparative Analysis of Genomic DNA Isolation Procedures: Hexadecyltrimethylammonium bromide (CTAB) and Liquid Detergent (Morning FreshR) methods from Samsorg 41 (ICSV 400)

H. M. Inuwa, I. A. Aimola, L. Inuwa
Journal Paper African Journal of Plant Science 5(4): 222 – 225.

Abstract

Expression of Mannose-Binding Insecticidal Lectin Gene in Transgenic Cotton (Gossypium) Plant.

H. M. Inuwa, N. B. Afolabi-Balogun, I. Sani, M. F. Ishiyaku, M. T. Bakare-Odunola, A. J. Nok L. van Emmenes
Journal Paper Cotton Genomics and Genetics (online) 2: 1-7. ISSN 1925-1947 http://cgg.sophiapublisher.com. 1-7

Abstract

Effect of Age on Transgenic Cotton (Gossypium) Plant Due to Expression of Mannose-Binding Lectin Gene from Allium sativum.

H. M. Inuwa, S. Sani, M. F. Ishiyaku, M. T. Bakare-Odunola, A. J. Nok, Lynelle van Emmenes, N. B. Afolabi-Balogun,
Journal Paper Asian Journal of Agricultural Sciences. 3 (5): 393 -396

Abstract

Trans- Sialidase-like gene from the Bloodstream form of Trypanosoma evansi conserves most of the Active Site Residues and Motifs Found in Trypanosoma Sialidases and Trans-Sialidases

H. M. Inuwa, Bitrus Yakubu, Andrew J. Nok, Ibrahim Sanni
Journal Paper African Journal of Biotechnology. 10 (13): 2388 – 2398.

Abstract

Cysteine Protease from Malaria Parasite, Plasmodium Berghei - Purification and Biochemical Characterisation

H. M. Inuwa, Emmanuel Amlabu, Andrew Jonathan Nok, Bukola Catherine Akin-Osanaiye, Emmanuel Haruna
Journal Paper Journal of Cell and Molecular Biology 9 (2): 43-49

Abstract

Screening Some Aspergillus Fungi for Cytosine Deaminase Activity.

H. M. Inuwa, H. Zanna, A. J. Nok, S. Ibrahim
Journal Paper International Journal of Biotechnology and Biochemistry 7(3): 397-403

Abstract

Sialic Acid Complement of Indigenous Nigerian Lizards and frogs.

H. M. Inuwa, Andrew Jonathan Nok, Isaac M. Onyenakasa, Nicodemus M. Useh
Journal Paper Comp. Clinical Pathology. DOI: 10.1007/s00580-011-1285-5 pg1-3.

Abstract

Comparative Determination of Anti nutritional Factors in Groundnut Oil and Palm Oil.

H. M. Inuwa, V. O. Aina, Baba Gabi, I. Aimola, Amao Toyin
Journal Paper Advance Journal of Food Science and Technology 3 (4): 275 – 279.

Abstract

Determination of Nephrotoxicity and Hepatotoxicity of Monosodium Glutamate (MSG) Consumption.

H. M. Inuwa, V. O. Aina, Baba Gabi, I. Aimola, Leehman Ja’afaru
Journal Paper British Journal of Pharmacology and Toxicology 2 (3): 148 - 153.

Abstract

Hypoglycemic Activity of Globimetulla browni Extracts in Streptozotocin-induced Diabetic Wistar Rats during Wet Season.

H. M. Inuwa, V. O. Aina, S. Ibrahim, D. A. Ameh
Journal PaperInternational Journal of Animal and Veterinary Advances 4 (1): 16 -18.

Abstract

Phytochemical Screening and Antibacterial Activity of Globimetulla browni Extracts during Wet Season.

H. M. Inuwa, V. O. Aina, S. Ibrahim, D. A. Ameh and M. R. Akinboboye
Journal Paper British Journal of Pharmacology and Toxicology 3 (1): 1 - 3

Abstract

Phytochemical Screening and Antibacterial Activity of Globimetulla browni Extracts during Dry Season

H. M. Inuwa, V. O. Aina, S. Ibrahim and D. A. Ameh
Journal Paper British Journal of Pharmacology and Toxicology 3 (1): 4 - 6.

Abstract

Isolation and Determination of Omega-9 Fatty Acids from Telfaria occidentalis.

H. M. Inuwa, I. A. AIMOLA, A. Muhamed, N. Habila, P. Okibe, M. Latayo and Z. Ahmed
Journal Paper International Journal of Food, Nutrition and Safety. 1 (1): 9 -14.

Abstract

Isolation and Determination of Omega-9 Fatty Acids from Telfaria occidentalis.

H. M. Inuwa, Nathan Habila, Idowu A. Aimola, Micheal U. Udeh, Emmanuel Haruna
Journal Paper Pathogenic mechanisms of Trypanosoma evansi Infections.” Research in Veterinary Science. 93. P 13-17

Abstract

Isolation and Determination of Omega-9 Fatty Acids from Telfaria occidentalis.

H. M. Inuwa, N. Habila, I. A. Aimola, O. I. Lasisi, A. Muhammad, A. I. Okafor, I. S. Williams
Journal PaperSegment Journals, Biological Segment 3 (1): BS/1565 1-10.

Abstract

Variation of Fatty Acids and Vitamin E Composition in Seed Oils of Some Plant Species.

H. M. Inuwa, N. Habila, I. A. Aimola, O. I. Lasisi, A. Muhammad, A. I. Okafor, I. S. Williams
Journal PaperSJournal of Plant Studies, 1 (2): 55-60.

Abstract

Correlation of acetylcholinesterase activity in the brain and blood of wistar rats acutely infected with Trypanosoma congolense.

H. M. Inuwa, I.A. Aimola, O. I.Lasisi, D.G. Chechet, I.A. Okafor, N. Habila
Journal PaperJournal of Acute Disease. 1: 26-30.

Abstract

Isolation and characterization of a mannose-binding insecticidal lectin gene fom Allium sativum (garlic) and its putative role in insect resistance using bioinformatics tools.

H. M. Inuwa, I. Sani, M. F. Ishiyaku, M. T. Bakare-Odunola, A. J. Nok, N. B. Afolabi-Balogun
Journal PaperInfection Genetics and Evolution. 12: 1508-1512

Abstract

Monocot mannose-binding lectins (MMBLs) or agglutinins are an extended superfamily of structurally and evolutionarily related proteins. They play important roles in plant defenses. Here we describe the synthesis of full-length cDNA of monocot mannose-binding insecticidal agglutinin isolated from Allium sativum, a traditional herb known to be of great applications in Africa, using reverse transcription polymerase chain reaction (RT-PCR) with specific primers designed based on the insecticidal sequence (NCBI primary accession no. AY866499.1). Sequence analysis revealed a 327bp open reading frame (ORF) encoding a putative 108-aa agglutinin precursor with a C-terminal domain. Multiple alignments of BLEC1 amino acids with those of eight other MMBLs revealed three highly conserved domains among them, indicating BLEC1 belongs to a member of the MMBL superfamily. Tertiary structure analysis showed that BLEC1 had three potential equal mannose-binding sites. Phylogenetic analysis indicated that 20 MMBLs including BLEC1 belonged to an extended superfamily. Gene ontology analyses indicate one biological process with GO ID: 0006952 representing defense response, with two secondary IDs GO: 0002217 GO: 0042829. The child terms has both negative and positive regulation some of which include GO: 0002242 defense response to parasitic plant and GO: 0002213 defense response to insect. The cloning and characterization of BLEC1 will enable us to study its potential use in plant genetic engineering in the development of insect resistance plant.

Purification and Characterization of Aspergillus parasiticus Cytosine Deaminase for Possible Deployment in Suicide Gene Therapy.

H. M. Inuwa, Hassan Zanna, A. J. Nok, S. Ibrahim,
Journal PaperAdvances in Biological Chemistry 2: 152-159.

Abstract

Cytosine Deaminase (CD) from Aspergillus parasiticus was purified and characterized. Time course for maximal CD production (50 µmol/min/mg) was at 72 hrs. The enzyme was purified 387.73 folds with an overall yield of 13%. The CD had pH optimum of 7.2, a temperature optimum of 40℃ - 45℃, activation of energy (Ea) of 8.4 KJ/mol and a t1/2 of 1.10 hr. A. parasiticus CD stoichiometrically deaminated Cyto-sine and 5-fluorocytosine (5-FC) with the KM values of 0.19mM and 0.30 mM respectively. Studies on the effect of pH on KM and Vmax gave pKa1 of 5.8 and pKa2 of 6.3 with enthalpy of ionization of 43.01 KJ/mole suggesting histidine in the active site. The enzyme was strongly inhibited by Ca2+, Co2+, Zn2+ and Hg2+ and completely inhibited by Cu2+ and Fe2+ at 50 mM. Therefore, A. parasiticus CD can be compared with CDs from other sources that are used in suicide gene therapy.

Isolation of Peripheral Blood Mononuclear Cells Using Glycerol Density Gradient.

H. M. Inuwa, A. J. Nok, A. I. Mamman, N. Habila,A. Muhammad, U. S. Ndidi, B. Ignatius,P. L. Jande, R. Oghor, I. A. Aimola, et al.
Journal PaperCell Biochem Biophys. DOI 10.1007/s12013-013-9744-z

Abstract

Ameliorative Effects of Acacia Honey against Sodium Arsenite-Induced Oxidative Stress in Some Viscera of Male Wistar Albino Rats

H. M. Inuwa, Muhammad Aliyu, Sani Ibrahim, Abdullahi B Sallau, Olagunju Abbas, Idowu A Aimola, Nathan Habila, Ndidi S Uche
Journal PaperBiochemistry Research International. 2013, Article ID 502438, 5 pages.

Abstract

Induction Of Foetal Haemoglobin Synthesis in Erythroid Progenitor Stem Cells: Mediated by Water‐Soluble Components of Terminalia Catappa

H. M. Inuwa, A. J. Nok, , A. I. Mamman, I. A. Aimola
Journal PaperCell Biochemistry and Function, 32(4), 361-367

Abstract

Lectures

  • Present 1995

    BTEC854: Agricultural Biotechnology

    Genetic transformation of plant and animal cells and generation of stably transformed organisms; Production of transgenic plants with resistance to pest, diseases or herbicides; Micro propagation techniques; Role of biotechnology in animal production and improvement of animal health; Aqua culture and biotechnology.

  • Present 2003

    SCI801: MANAGEMENT AND ENTREPRENEURSHIP

    Teaching Management and Entrepreneurship skills related to the field of Biotechnology.

  • Present 2010

    BCHM811: Biotechnology and Genetic Engineering

    Principles and techniques of genetic engineering recombinant DNA technology, DNA vector and purification, Restriction endonucleases and their application, preparation of the genetic libraries using phage vectors, sub cloning with plasmid vector. Gene enrichment and application, strain improvement through mutant selection and recombinations. Role of microorganism in the production of biomaterials and extraction & recovery of metal production of industrial chemicals, e.g ethanol. Enzyme technology. Production of monoclonal antibodies. Role of microbial activity in food production. Application of biotechnology in crop improvement, medicine and drug production.

  • Present 2010

    BCHM830: Technical Paper Writing

    Scientific information sourcing; scientific paper writing. The title, introduction, objective, materials and methods, results, discussion, presentation of tables and figures. How to prepare and cite reference. Types of scientific writing. The laboratory report. Paper publication for conferences and journal, review articles, seminar paper. Thesis writing, research proposals. Scientific language; the use and music of English. Acknowledgement, rights and permissions.

Specialisation

  • Date 2003

    Bioinformatics

    The science of collecting and analyzing complex biological data such as genetic codes.

  • Date 1989

    Plant Gene Expression

    Study of Plant DNA.

  • Date 1979

    Clinical Biochemistry

    Clinical biochemistry addresses the functioning of normal and diseased organisms from a biochemical point of view.

  • Date 1989

    Biotechonology

    Biotechnology is the use of living systems and organisms to develop products for a specific purpose.

At My Office

You can find me at my office located at the Biochemistry Department, Ahmadu Bello University.

I am at my office every day from 9:00 until 17:00, but you may consider a call to fix an appointment.

At My Lab

You can find me at the lab located on the first floor of the Biochemistry Department, Ahmadu Bello University.

I am usually at the lab if there are practicals or research work to be done. It is best if you try to contact me in the office.